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KMID : 1189420200330030001
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2020 Volume.33 No. 3 p.1 ~ p.26
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Effects of Three Types of GagamBangpungtongseong-san(Except Talcum) on the Atopic Dermatitis in Mice
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Lim Kyeong-Min
Song Ji-Hoon
Kim Jong-Han
Choi Jung-Hwa
Jung Min-Yeong
Park Soo-Yeon
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Abstract
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Objectives : This study was to examine the effects of 3 types of BTS which were excluded talcum only or replaced talcum to Lonicera japonicae Flos or Kochiae Fructus on the DNCB-induced atopic dermatitis in mice.
Methods : In this study, Balb/c mice were divided into five groups: normal, control, GBT(BTS except talcum), GBTG(GBT added Lonicera japonicae Flos), and GBTJ(GBT added Kochiae Fructus). And the effects on atopic dermatitis were evaluated by weight change, ear’s thickness and weight, thickness of dorsal skin, severity scale of dorsal skin, histopathologic findings of dorsal skin by H&E and toluidine blue stain, proliferation of splenocyte and thymocyte in vitro, proliferation of splenocyte in vivo, IL-4, TNF-α, IgE in serum.
Results : There were no significantly changes in body weight and effect of ear’s weight in GBT, GBTG, and GBTJ group. The thickness of ear of GBT and GBTJ group showed significant decrease. And the thickness of dorsal skin of GBTJ group significantly decreased compared to the control, GBT, and GBTG group. All the treated groups significantly decreased in severity scale, histopathologically reduced epidermal thickness, and mast cell infiltration. In vitro, all the treated groups increased in the proliferation rates of splenocyte. However, in vivo study, it showed a falling tendency and GBT group significantly decreased compared to control, GBTG, and GBTJ group. In vitro study, GBTG group significantly decreased in the proliferation rates of thymocyte. There was no IgE contents chnage in GBT, GBTG, and GBTJ groups but IL-4 and TNF-α contents were significantly decreased.
Conclusions : GBT, GBTG, and GBTJ are expected to improve symptoms of atopic dermatitis and further studies are needed for development of BTS’s transformation.
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KEYWORD
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Atopic Dermatitis, Bangpungtongseong-san, talcum, Lonicera japonicae Flos, Kochiae Fructus, 2,4-dinitrochlorobenzene(DNCB)
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